Part:BBa_K3970028

LexO-cpTDH3-CpcE-tCYC1

Usage and Biology

This part is the effector of the upstream regulator, the product of the LexA will bind to the following LexO part and active the downstream cpcE gene expression. With the regulation of cpcE, the lyase a-subunit will not effect the growth of yeast.

Construction

Sequence

CTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGTTGCTTTGAACGACCCATTCATCACCAACGACTACGCTGCTTACATGTTCAAGTACGACTCCACTCACGGTAGATACGCTGGTGAAGTTTCCCACGATGACAAGCACATCATTGTCGATGGTAAGAAGATTGCTACTTACCAAGAAAGAGACCCAGCTAACTTGCCATGGGGTTCTTCCAACGTTGACATCGCCATTGACTCCACTGGTGTTTTCAAGGAATTAGACACTGCTCAAAAGCACATTGACGCTGGTGCCAAGAAGGTTGTTATCACTGCTCCATCTTCCACCGCCCCAATGTTCGTCATGGGTGTTAACGAAGAAAAATACACTTCTGACTTGAAGATTGTTTCCAACGCTTCTTGTACCACCAACTGTTTGGCTCCATTGGCCAAGGTTATCAACGATGCTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCTTTGACTGCTACTCAAAAGACTGTTGACGGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACCGCTTCCGGTAACATCATCCCATCCTCCACCGGTGCTGCTAAGGCTGTCGGTAAGGTCTTGCCAGAATTGCAAGGTAAGTTGACCGGTATGGCTTTCAGAGTCCCAACCGTCGATGTCTCCGTTGTTGACTTGACTGTCAAGTTGAACAAGGAAACCACCTACGATGAAATCAAGAAGGTTGTTAAGGCTGCCGCTGAAGGTAAGTTGAAGGGTGTTTTGGGTTACACCGAAGACGCTGTTGTCTCCTCTGACTTCTTGGGTGACTCTCACTCTTCCATCTTCGATGCTTCCGCTGGTATCCAATTGTCTCCAAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTACCAGAGTTGTCGACTTGGTTGAACACGTTGCCAAGGCTTAAATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAAGGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACGGTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGTTTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCATCCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTTGGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGAtggtccgagtcatgtaattagttatgtcacgcttacGttcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgc

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]